Abstract
Degenerate DNA oligomers coding for highly conserved regions of the voltage-gated calcium channel were synthesized for the polymerase chain reaction (PCR) using DNA from a human brain cDNA library as template. PCR amplified a 640-bp DNA fragment from the human brain cDNA library. Sequencing revealed that this fragment encodes part of a protein highly homologous to a subtype of the dihydropyridine-sensitive calcium channel cloned from rabbit heart and rat brain. Southern analysis of panels of somatic cell hybrids mapped the 640-bp fragment, CACNL1A1, to human chromosome 12p13-pter.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Southern
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Brain / metabolism*
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Calcium Channels / genetics*
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Calcium Channels, L-Type
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Chromosome Mapping
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Chromosomes, Human, Pair 12*
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Cricetinae
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DNA
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Humans
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Hybrid Cells
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Ion Channel Gating
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Molecular Sequence Data
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Nerve Tissue Proteins / genetics*
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Polymerase Chain Reaction
Substances
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CACNA1C protein, human
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Calcium Channels
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Calcium Channels, L-Type
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Nerve Tissue Proteins
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DNA
Associated data
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GENBANK/L04150
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GENBANK/L04151
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GENBANK/M88363
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GENBANK/M88364
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GENBANK/M88365
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GENBANK/M88366
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GENBANK/M90549
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GENBANK/M90550
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GENBANK/M90551
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GENBANK/M91370