Abstract
We have discovered a protein termed Dr1 that interacts with the TATA-binding protein, TBP. The association of Dr1 with TBP results in repression of both basal and activated levels of transcription. The interaction of Dr1 with TBP precludes the formation of a transcription-competent complex by inhibiting the association of TFIIA and/or TFIIB with TBP. Dr1 activity is associated with a 19 kd protein. A cDNA clone encoding Dr1 was isolated. Dr1 is phosphorylated in vivo and phosphorylation of Dr1 affected its interaction with TBP. Our results suggest a regulatory role for Dr1 in repression of transcription mediated via phosphorylation.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Blotting, Western
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Cloning, Molecular
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DNA
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DNA-Binding Proteins / metabolism*
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Electrophoresis, Polyacrylamide Gel
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Escherichia coli
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Gene Expression Regulation*
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Genes, MHC Class II*
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HeLa Cells
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Humans
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Molecular Sequence Data
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Phosphoproteins / genetics
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Phosphoproteins / metabolism*
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Phosphorylation
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Precipitin Tests
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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TATA-Box Binding Protein
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Transcription Factor TFIIA
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Transcription Factor TFIIB
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Transcription, Genetic*
Substances
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DNA-Binding Proteins
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Phosphoproteins
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Recombinant Proteins
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TATA-Box Binding Protein
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Transcription Factor TFIIA
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Transcription Factor TFIIB
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Transcription Factors
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down-regulator of transcription 1
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DNA
Associated data
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GENBANK/L05499
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GENBANK/M87278
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GENBANK/M94130
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GENBANK/M94264
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GENBANK/M97388
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GENBANK/S72766
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GENBANK/S72767
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GENBANK/S72768
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GENBANK/S72769
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GENBANK/S72771