Analysis of ras oncogene mutations in human squamous cell carcinoma of the head and neck

F Núñez; O Domínguez; E Coto; C Suárez-Nieto; P Pérez; C López-Larrea

doi:10.1016/0960-7404(92)90043-k

Analysis of ras oncogene mutations in human squamous cell carcinoma of the head and neck

Surg Oncol. 1992 Dec;1(6):405-11. doi: 10.1016/0960-7404(92)90043-k.

Authors

F Núñez¹, O Domínguez, E Coto, C Suárez-Nieto, P Pérez, C López-Larrea

Affiliation

¹ Department of Otolaryngology, Hospital Central de Asturias, Oviedo, Spain.

PMID: 1341278
DOI: 10.1016/0960-7404(92)90043-k

Abstract

The presence of proto-oncogene mutations at codons 12, 13 and 61 of the Ha-, Ki-, and N-ras in primary head and neck squamous cell carcinoma are analysed in this study. Oncogene ras-specific sequences were amplified by the polymerase chain reaction and probed with mutation specific oligonucleotide probes. Mutations were detected in 8 of 22 samples (36.3%). No mutations were detected on patients' peripheral blood DNA. We found that histologically and clinically, squamous cell carcinomas with or without a ras mutation do not differ significantly from each other.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carcinoma, Squamous Cell / genetics*
Carcinoma, Squamous Cell / mortality
Carcinoma, Squamous Cell / pathology
Chi-Square Distribution
DNA / genetics
Genes, ras / genetics*
Head and Neck Neoplasms / genetics*
Head and Neck Neoplasms / mortality
Head and Neck Neoplasms / pathology
Humans
Mutation / genetics*
Neoplasm Staging
Nucleic Acid Hybridization
Oligonucleotide Probes
Polymerase Chain Reaction
Proto-Oncogene Mas
Spain / epidemiology
Survival Analysis

Substances

MAS1 protein, human
Oligonucleotide Probes
Proto-Oncogene Mas
DNA