Differential induction of immediate early genes by excitatory amino acid receptor types in primary cultures of cortical and striatal neurons

Brain Res Mol Brain Res. 1992 Jan;12(1-3):233-41. doi: 10.1016/0169-328x(92)90089-t.

Abstract

In primary cultures of neurons from cerebral cortex and striatum, 30 s stimulation with the excitatory amino acid glutamate elicited a 5 to 9-fold increase in immediate early gene (IEG) mRNAs. Glutamate increased c-fos, c-jun, jun-B, and NGFI-A (zif/268) mRNAs by binding to both alpha-amino-3-hydroxy-5-methylisoxazolepropionic acid (AMPA) and N-methyl-D-aspartate (NMDA) receptor types, and increased c-fos, jun-B, and NGFI-A mRNAs by binding to the metabotropic receptor. NMDA receptor activation elicited IEG expression by a transmembrane calcium influx; AMPA receptor-induced depolarization played a permissive role for the opening of the NMDA receptor channel. The protein kinase C (PKC) inhibitor H-7 (but not inhibitors of cyclic nucleotide-dependent and calcium/calmodulin-dependent protein kinases) partially blocked IEG expression induced by glutamate.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
  • 6-Cyano-7-nitroquinoxaline-2,3-dione
  • Animals
  • Animals, Newborn
  • Blotting, Northern
  • Cells, Cultured
  • Cerebral Cortex / physiology*
  • Corpus Striatum / physiology*
  • DNA-Binding Proteins / genetics
  • Early Growth Response Protein 1
  • Gene Expression Regulation*
  • Genes, Regulator*
  • Genes, fos / drug effects
  • Genes, jun / drug effects
  • Glutamates / pharmacology*
  • Glutamic Acid
  • Glycine / pharmacology*
  • Immediate-Early Proteins*
  • Isoquinolines / pharmacology
  • Neurons / drug effects
  • Neurons / physiology*
  • Piperazines / pharmacology
  • Protein Kinase Inhibitors
  • Proto-Oncogenes* / drug effects
  • Quinoxalines / pharmacology*
  • Quisqualic Acid / pharmacology*
  • RNA, Messenger / biosynthesis*
  • RNA, Messenger / genetics
  • Rats
  • Receptors, AMPA
  • Receptors, N-Methyl-D-Aspartate / drug effects
  • Receptors, N-Methyl-D-Aspartate / physiology*
  • Receptors, Neurotransmitter / drug effects
  • Receptors, Neurotransmitter / physiology*
  • Sulfonamides / pharmacology
  • Transcription Factors / genetics

Substances

  • DNA-Binding Proteins
  • Early Growth Response Protein 1
  • Egr1 protein, rat
  • Glutamates
  • Immediate-Early Proteins
  • Isoquinolines
  • Piperazines
  • Protein Kinase Inhibitors
  • Quinoxalines
  • RNA, Messenger
  • Receptors, AMPA
  • Receptors, N-Methyl-D-Aspartate
  • Receptors, Neurotransmitter
  • Sulfonamides
  • Transcription Factors
  • Glutamic Acid
  • W 7
  • 6-Cyano-7-nitroquinoxaline-2,3-dione
  • 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
  • Quisqualic Acid
  • Glycine