Abstract
Expression of the platelet-derived growth factor (PDGF) A-chain gene is known to give rise to multiple transcripts of different sizes. Northern blot analysis, using a set of DNA probes corresponding to various parts of the human PDGF A-chain gene, indicated heterogeneity in both the 5' and 3' end of the transcripts. The 3' heterogeneity was shown to be the result of differential use of three polyadenylation signals. S1-nuclease protection- and chloramphenicol acetyltransferase (CAT)-assays, revealed the 5' heterogeneity to be the consequence of two alternative promoters. Whereas the upstream promoter contained typical TATA- and CAAT-boxes, the downstream promoter lacked a TATA-box but seemed to be composed of several GC-boxes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alternative Splicing*
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Base Sequence
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Chloramphenicol O-Acetyltransferase / biosynthesis
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Chloramphenicol O-Acetyltransferase / genetics
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Chromosome Mapping
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DNA Probes
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Exons
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Glioma
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Humans
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Macromolecular Substances
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Molecular Sequence Data
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Oligodeoxyribonucleotides
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Platelet-Derived Growth Factor / biosynthesis
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Platelet-Derived Growth Factor / genetics*
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Poly A / genetics*
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Poly A / metabolism
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Polymerase Chain Reaction / methods
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Promoter Regions, Genetic*
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RNA, Messenger / genetics*
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RNA, Messenger / metabolism
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RNA, Neoplasm / genetics
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RNA, Neoplasm / isolation & purification
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RNA, Neoplasm / metabolism
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Recombinant Fusion Proteins / biosynthesis
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Transcription, Genetic
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Transfection
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Tumor Cells, Cultured
Substances
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DNA Probes
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Macromolecular Substances
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Oligodeoxyribonucleotides
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Platelet-Derived Growth Factor
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RNA, Messenger
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RNA, Neoplasm
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Recombinant Fusion Proteins
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Poly A
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Chloramphenicol O-Acetyltransferase
Associated data
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GENBANK/M64870
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GENBANK/M64871
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GENBANK/M64872
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GENBANK/M64873
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GENBANK/M64874
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GENBANK/M64875
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GENBANK/M64876
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GENBANK/M64877
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GENBANK/S50869
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GENBANK/S51624