Sulmazole effects on PGE2 and D-Ala2-Met-enkephalinamide modulation of cyclic AMP synthesis and neurotransmitter release in a sympathetic ganglion

Neurosci Lett. 1992 Sep 28;145(1):105-8. doi: 10.1016/0304-3940(92)90214-r.

Abstract

In the guinea-pig superior cervical ganglion, the Gi blocking agent sulmazole enhanced the basal and prostaglandin E2-induced stimulation of cyclic AMP synthesis but had no effect on the prostaglandin-dependent inhibition of acetylcholine release. On the contrary sulmazole counteracted the inhibitory effect of D-Ala2-Met-enkephalinamide both on cyclic AMP formation and acetylcholine release. Moreover sulmazole eliminated the supra-additive effect of the combination of prostaglandin + opiate on cyclic AMP synthesis. The presence of a Gi-coupled opiate receptor at the pre-and postsynaptic levels is suggested.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylcholine / metabolism
  • Adenylyl Cyclases / metabolism
  • Adipose Tissue / drug effects
  • Animals
  • Cardiotonic Agents / pharmacology*
  • Cyclic AMP / biosynthesis*
  • Dinoprostone / pharmacology*
  • Dose-Response Relationship, Drug
  • Enkephalin, Methionine / analogs & derivatives*
  • Enkephalin, Methionine / pharmacology
  • GTP-Binding Proteins / metabolism
  • Ganglia, Sympathetic / drug effects
  • Ganglia, Sympathetic / metabolism*
  • Guinea Pigs
  • Imidazoles / pharmacology*
  • In Vitro Techniques
  • Kinetics
  • Neurotransmitter Agents / metabolism*

Substances

  • Cardiotonic Agents
  • Imidazoles
  • Neurotransmitter Agents
  • Enkephalin, Methionine
  • enkephalinamide-Met, Ala(2)-
  • Cyclic AMP
  • GTP-Binding Proteins
  • Adenylyl Cyclases
  • sulmazole
  • Dinoprostone
  • Acetylcholine