Abstract
The penDE gene from Penicillium chrysogenum has been isolated; the gene is located in close vicinity of the pcbC gene. Amplification of the pcbC-penDE gene cluster in Penicillium chrysogenum Wis54-1255 leads to a significant increase in penicillin production. In selected transformants an increase of up to 40% is observed.
MeSH terms
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Acyltransferases / biosynthesis*
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Acyltransferases / genetics
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Drug Resistance, Microbial
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Enzyme Induction
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Fungal Proteins / biosynthesis*
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Fungal Proteins / genetics
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Gene Amplification
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Gene Expression Regulation, Fungal
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Genes, Fungal*
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Introns
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Penicillin V / metabolism*
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Penicillin-Binding Proteins*
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Penicillium chrysogenum / genetics*
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Penicillium chrysogenum / metabolism
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Phleomycins / pharmacology
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Recombinant Fusion Proteins / biosynthesis*
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Recombinant Fusion Proteins / genetics
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Selection, Genetic
Substances
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Fungal Proteins
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Penicillin-Binding Proteins
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Phleomycins
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Recombinant Fusion Proteins
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Acyltransferases
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acyl-CoA-6-aminopenicillanic acid acyltransferase
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Penicillin V