Abstract
The hypothesis of astroglial cell involvement in prenatal setting up of the blood-brain barrier (BBB) has been examined by producing glial degeneration in cerebellum of chicken embryos submitted to the action of gliotoxin 6-aminonicotinamide (6-AN), which was applied onto the embryo chorioallantoic membrane during both early and late embryonic development. The effects of 6-AN on the cerebellum astroglial cells and microvessels were analysed under the light microscope by immunostaining for 3CB2 (chick-specific glial marker) and HT7 (chick-specific marker of BBB-provided brain endothelia), under the electron microscope, as well as by the vascular permeability tracer horseradish peroxidase. The results, showing good suitability of the 6-AN model also when applied in early embryonic development, demonstrated a correlation between perivascular glia depletion and endothelial barrier impairment and suggested that astroglia play a role in the BBB prenatal differentiation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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6-Aminonicotinamide / pharmacology*
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Animals
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Antigens, CD*
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Antigens, Neoplasm*
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Antigens, Surface*
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Astrocytes / drug effects
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Astrocytes / physiology*
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Avian Proteins*
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Basigin
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Blood Proteins*
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Blood-Brain Barrier / cytology
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Blood-Brain Barrier / drug effects
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Blood-Brain Barrier / embryology*
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Blood-Brain Barrier / physiology
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Cell Differentiation / drug effects
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Cerebellum / blood supply
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Cerebellum / drug effects
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Cerebellum / embryology*
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Chick Embryo
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Endothelium, Vascular / cytology
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Endothelium, Vascular / embryology
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Horseradish Peroxidase / metabolism
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Immunohistochemistry
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Membrane Glycoproteins / analysis
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Microcirculation / embryology*
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Microcirculation / ultrastructure
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Neuroglia / chemistry
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Neuroglia / cytology
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Neuroglia / drug effects
Substances
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Antigens, CD
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Antigens, Neoplasm
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Antigens, Surface
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Avian Proteins
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Blood Proteins
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Bsg protein, Gallus gallus
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Bsg protein, rat
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Membrane Glycoproteins
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Basigin
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6-Aminonicotinamide
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Horseradish Peroxidase