Abstract
The c-ets1 proteins are transcriptional activators expressed within endothelial cells during blood vessel development in chick embryos. The authors show by in situ hybridization that c-ets1 is transcribed in the endothelia during angiogenesis in human embryos, in granulation tissue, and especially during tumor vascularization. c-ets1 mRNAs were also detected in the fibrocytes of tumor stroma and in the spindle cells of Kaposi's sarcomas, regarded as cells of endothelial origin. It has been shown that the c-ets proteins activate transcription through a PEA3 motif that plays a role in the stimulation of transcription of urokinase-type plasminogen-activator (u-PA), stromelysin and collagenase genes. The authors demonstrate in vitro that the angiogenic factor TNF alpha increases transiently the amount of both c-ets1 and u-PA mRNA in confluent human umbilical vein endothelial cells. Therefore, the authors suggest that the c-ets1 proteins might regulate the transcription of the genes coding for matrix-degrading proteases, which are necessary for both angiogenesis and tumor invasion.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenocarcinoma / chemistry
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Adenocarcinoma / genetics
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Adenocarcinoma / pathology
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Adolescent
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Adult
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Aged
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Carcinoma, Small Cell / chemistry
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Carcinoma, Small Cell / genetics
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Carcinoma, Small Cell / pathology
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Carcinoma, Transitional Cell / chemistry
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Carcinoma, Transitional Cell / genetics
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Carcinoma, Transitional Cell / pathology
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Cells, Cultured
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Child
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Embryo, Mammalian / chemistry
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Endothelium, Vascular / chemistry
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Endothelium, Vascular / pathology*
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Female
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Granulation Tissue / chemistry
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Humans
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Kidney Neoplasms / chemistry
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Kidney Neoplasms / genetics
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Kidney Neoplasms / pathology
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Lung Neoplasms / chemistry
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Lung Neoplasms / genetics
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Lung Neoplasms / pathology
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Male
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Matrix Metalloproteinase 3
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Metalloendopeptidases / genetics
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Microbial Collagenase / genetics
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Neovascularization, Pathologic*
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Nucleic Acid Hybridization
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Proto-Oncogene Mas
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Proto-Oncogene Protein c-ets-1
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Proto-Oncogene Proteins / analysis
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Proto-Oncogene Proteins / genetics*
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Proto-Oncogene Proteins c-ets
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RNA, Messenger / analysis
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RNA, Messenger / genetics
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Sarcoma, Kaposi / chemistry
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Sarcoma, Kaposi / genetics
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Sarcoma, Kaposi / pathology
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Skin Neoplasms / chemistry
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Skin Neoplasms / genetics
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Skin Neoplasms / pathology
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Transcription Factors / analysis
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Transcription Factors / genetics*
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Transcription, Genetic / genetics*
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Urinary Bladder Neoplasms / chemistry
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Urinary Bladder Neoplasms / genetics
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Urinary Bladder Neoplasms / pathology
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Urokinase-Type Plasminogen Activator / genetics
Substances
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ETS1 protein, human
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MAS1 protein, human
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Proto-Oncogene Mas
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Proto-Oncogene Protein c-ets-1
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-ets
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RNA, Messenger
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Transcription Factors
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Urokinase-Type Plasminogen Activator
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Metalloendopeptidases
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Matrix Metalloproteinase 3
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Microbial Collagenase