Abstract
The receptors that mediate monocyte adhesion to cytokine-stimulated endothelial monolayers were assessed using a nonstatic (rotating) cell-attachment assay. In this system, leukocyte adhesion molecule-1 (LAM-1) (L-selectin) mediated a major portion (87 +/- 15% at 37 degrees C) of monocyte attachment to activated endothelium. mAb blocking of endothelial leukocyte adhesion molecule-1 (41% inhibition), CD18 (36%), and vascular cell adhesion molecule-1 (25%) function had lesser effects on attachment. These results suggest that LAM-1 may serve an important role in monocyte attachment to endothelium at sites of inflammation.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antibodies, Monoclonal
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Antigens, CD / physiology
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CD18 Antigens
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Cell Adhesion Molecules / biosynthesis
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Cell Adhesion Molecules / immunology
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Cell Adhesion Molecules / physiology*
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Cell Adhesion* / drug effects
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Cell Line
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Endothelium, Vascular / drug effects
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Endothelium, Vascular / physiology*
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Humans
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L-Selectin
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Monocytes / drug effects
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Monocytes / physiology*
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Receptors, Leukocyte-Adhesion / physiology
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Tumor Necrosis Factor-alpha / drug effects
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Umbilical Veins
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Vascular Cell Adhesion Molecule-1
Substances
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Antibodies, Monoclonal
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Antigens, CD
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CD18 Antigens
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Cell Adhesion Molecules
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Receptors, Leukocyte-Adhesion
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Tumor Necrosis Factor-alpha
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Vascular Cell Adhesion Molecule-1
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L-Selectin