Glucagonlike peptide-1(7-36)amide suppresses glucagon secretion and decreases cyclic AMP concentration in cultured In-R1-G9 cells

Biochem Biophys Res Commun. 1992 Jul 15;186(1):503-8. doi: 10.1016/s0006-291x(05)80836-8.

Abstract

We previously reported that GLP-1(7-36)amide had glucagonostatic action as well as insulinotropic action in the perfused rat pancreas. In this study, we examined the effect of GLP-1(7-36)amide on glucagon secretion and cAMP concentration in glucagon-secreting cell line, In-R1-G9. GLP-1(7-36)amide (1nM) significantly suppressed glucagon secretion and decreased cAMP concentration in the cells. GLP-1(1-37) did not affect glucagon secretion. It is suggested that inhibitory effect of GLP-1(7-36)amide on glucagon secretion is at least partly mediated by adenylate cyclase system.

Publication types

  • Comparative Study

MeSH terms

  • 1-Methyl-3-isobutylxanthine / pharmacology
  • Animals
  • Clone Cells
  • Cricetinae
  • Cyclic AMP / metabolism*
  • Dose-Response Relationship, Drug
  • Glucagon / antagonists & inhibitors
  • Glucagon / metabolism*
  • Glucagon-Like Peptide 1
  • Glucagon-Like Peptides
  • Insulinoma / metabolism
  • Kinetics
  • Pancreatic Neoplasms / metabolism
  • Peptide Fragments / pharmacology*
  • Peptides / pharmacology*

Substances

  • Peptide Fragments
  • Peptides
  • glucagon-like peptide 1 (7-36)
  • Glucagon-Like Peptides
  • glucagon-like peptide 1 (1-37)
  • Glucagon-Like Peptide 1
  • Glucagon
  • Cyclic AMP
  • 1-Methyl-3-isobutylxanthine