Specific detection of c-erbB-2 mRNA expression in gastric cancers by the polymerase chain reaction following reverse transcription

I Ninomiya; Y Endo; Y Yonemura; M Noguchi; S Fushida; M Nakai; H Takamura; F Harada; T Suzuki; I Miyazaki

doi:10.1038/bjc.1992.221

Specific detection of c-erbB-2 mRNA expression in gastric cancers by the polymerase chain reaction following reverse transcription

Br J Cancer. 1992 Jul;66(1):84-7. doi: 10.1038/bjc.1992.221.

Authors

I Ninomiya¹, Y Endo, Y Yonemura, M Noguchi, S Fushida, M Nakai, H Takamura, F Harada, T Suzuki, I Miyazaki, et al.

Affiliation

¹ Department of Surgery II, School of Medicine, Kanazawa University, Japan.

Abstract

The expression of the c-erbB-2 mRNA in human embryonic lung fibroblasts, a gastric cancer cell line, mature placenta, and 25 gastric cancer tissues was examined by using the polymerase chain reaction following reverse transcription. This technique can be used to examine c-erbB-2 mRNA expression in a small endoscopic biopsy specimen before surgery.

MeSH terms

Actins / genetics
Base Sequence
Blotting, Southern
Cell Line
Humans
Molecular Sequence Data
Oligodeoxyribonucleotides
Oligonucleotides, Antisense
Polymerase Chain Reaction / methods
Protein-Tyrosine Kinases / genetics
Proto-Oncogene Proteins / genetics*
Proto-Oncogenes*
RNA, Messenger / genetics*
RNA, Messenger / isolation & purification
RNA, Messenger / metabolism
RNA-Directed DNA Polymerase
Receptor, ErbB-2
Stomach Neoplasms / genetics*

Substances

Actins
Oligodeoxyribonucleotides
Oligonucleotides, Antisense
Proto-Oncogene Proteins
RNA, Messenger
Protein-Tyrosine Kinases
Receptor, ErbB-2
RNA-Directed DNA Polymerase