Abstract
A Src homology 3 (SH3) region is a sequence of approximately 50 amino acids found in many nonreceptor tyrosine kinases and other proteins. Deletion of the SH3 region from the protein encoded by the c-abl proto-oncogene activates the protein's transforming capacity, thereby suggesting the participation of the SH3 region in the negative regulation of transformation. A complementary DNA was isolated that encoded a protein, 3BP-1, to which the SH3 region of Abl bound with high specificity and to which SH3 regions from other proteins bound differentially. The sequence of the 3BP-1 protein is similar to that of a COOH-terminal segment of Bcr and to guanosine triphosphatase-activating protein (GAP)-rho, which suggests that it might have GAP activity for Ras-related proteins. The 3BP-1 protein may therefore be a mediator of SH3 function in transformation inhibition and may link tyrosine kinases to Ras-related proteins.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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3T3 Cells
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Amino Acid Sequence
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Animals
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Binding Sites
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Chimera
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Cloning, Molecular
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GTPase-Activating Proteins
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Gene Library
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Genes, abl*
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Genes, src*
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Glutathione Transferase / genetics
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Glutathione Transferase / metabolism
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Mice
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Molecular Sequence Data
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Oncogene Proteins / genetics
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Oncogene Proteins / metabolism*
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Plasmids
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Polymerase Chain Reaction / methods
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Prosencephalon / physiology
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Protein-Tyrosine Kinases / metabolism*
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Proteins / metabolism*
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Proto-Oncogene Proteins c-abl / genetics
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Proto-Oncogene Proteins c-abl / metabolism*
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Proto-Oncogene Proteins c-bcr
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Proto-Oncogene Proteins pp60(c-src) / genetics
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Proto-Oncogene Proteins pp60(c-src) / metabolism*
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Proto-Oncogene Proteins*
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Recombinant Fusion Proteins / metabolism
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Restriction Mapping
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Rho Factor / metabolism*
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Sequence Homology, Nucleic Acid
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ras GTPase-Activating Proteins
Substances
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GTPase-Activating Proteins
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Oncogene Proteins
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Proteins
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Proto-Oncogene Proteins
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Recombinant Fusion Proteins
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Rho Factor
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ras GTPase-Activating Proteins
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Glutathione Transferase
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Protein-Tyrosine Kinases
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Proto-Oncogene Proteins c-abl
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Proto-Oncogene Proteins pp60(c-src)
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Bcr protein, mouse
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Proto-Oncogene Proteins c-bcr