The detection of Mycobacterium tuberculosis in uncultured clinical specimens using the polymerase chain reaction and a non-radioactive DNA probe

D Thierry; C Chureau; C Aznar; J L Guesdon

doi:10.1016/0890-8508(92)90015-p

The detection of Mycobacterium tuberculosis in uncultured clinical specimens using the polymerase chain reaction and a non-radioactive DNA probe

Mol Cell Probes. 1992 Jun;6(3):181-91. doi: 10.1016/0890-8508(92)90015-p.

Authors

D Thierry¹, C Chureau, C Aznar, J L Guesdon

Affiliation

¹ Laboratoire des Sondes Froides, Institut Pasteur, Paris, France.

PMID: 1383698
DOI: 10.1016/0890-8508(92)90015-p

Abstract

A Sal I-Hin dIII restriction fragment from Mycobacterium tuberculosis was found to hybridize specifically with genomic DNA from M. tuberculosis. Primers were designed from the sequence of this fragment and used to amplify uniquely M. tuberculosis-group DNA in a polymerase chain reaction. It is suggested that a combination of these primers and an acetylaminofluorene-labelled probe will prove to be a useful tool for the early diagnosis of tuberculous infections.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Body Fluids / microbiology
DNA Probes*
DNA, Bacterial / analysis
Humans
Molecular Sequence Data
Mycobacterium avium / genetics
Mycobacterium bovis / genetics
Mycobacterium tuberculosis / genetics
Mycobacterium tuberculosis / isolation & purification*
Polymerase Chain Reaction*
Predictive Value of Tests
Sensitivity and Specificity
Staining and Labeling
Tuberculosis / diagnosis
Tuberculosis / microbiology*

Substances

DNA Probes
DNA, Bacterial