Characterization of monoclonal antibodies against Actinobacillus pleuropneumoniae serotype 5

Am J Vet Res. 1992 Sep;53(9):1519-23.

Abstract

Two monoclonal antibodies against Actinobacillus pleuropneumoniae serotype 5, designated as 5MAb-1 and 5MAb-6, were characterized. Enzyme-linked immunosorbent assay-inhibition tests with whole-cell antigens obtained from strains of serotype 1 through 12 of A pleuropneumoniae revealed that 5 MAb-1 bound to only serotype-5 strains. The epitope recognized by 5MAb-1 was a carbohydrate that was sensitive to periodate oxidation and resided on the structure of beta-1,6-linked D-galactose in an O-antigen polysaccharide of serotype-5 lipopolysaccharide. Analysis of these results revealed that the O-antigen polysaccharide of lipopolysaccharide was 1 of the antigenic determinants responsible for the serotype specificity of A pleuropneumoniae. On the other hand, 5MAb-6 reacted with strains of serotype 1 through 10 in varying degrees and its epitope was located on polypeptides sensitive to proteinase K. In an immunoblotting analysis, 5MAb-6 reacted with 2 polypeptide bands, with molecular weights of approximately 41,500 and 28,000, in the outer membrane protein-rich fraction obtained from strains of serotype 1 through 10. These results indicated that outer membrane proteins from several serotype strains of A pleuropneumoniae possessed common antigenic determinants.

MeSH terms

  • Actinobacillus pleuropneumoniae / classification
  • Actinobacillus pleuropneumoniae / immunology*
  • Animals
  • Antibodies, Bacterial / immunology*
  • Antibodies, Monoclonal / biosynthesis
  • Antibodies, Monoclonal / immunology*
  • Antigens, Bacterial / immunology*
  • Bacterial Outer Membrane Proteins / immunology
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes / immunology
  • Hybridomas
  • Immunoblotting
  • Lipopolysaccharides / immunology
  • Polysaccharides, Bacterial / immunology
  • Serotyping
  • Swine

Substances

  • Antibodies, Bacterial
  • Antibodies, Monoclonal
  • Antigens, Bacterial
  • Bacterial Outer Membrane Proteins
  • Epitopes
  • Lipopolysaccharides
  • Polysaccharides, Bacterial