A comparison of two autoradiographic methods for detecting radiolabeled nucleic acids in embryos

Mol Reprod Dev. 1992 Oct;33(2):141-8. doi: 10.1002/mrd.1080330205.

Abstract

Two methods for preparing embryos for autoradiographic study of newly synthesized nucleic acids are described and compared. The first method consists of rapidly fixing radiolabeled embryos with acetic acid:methanol, spreading them on glass slides and exposing them for 8 days with a photographic emulsion. The second method consists of fixing, embedding in resin, and sectioning the embryos before their exposure with the emulsion for 3 weeks. Both techniques have many applications in studies of early embryonic activity, but the spread technique is very sensitive, simpler, and faster.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Autoradiography / methods*
  • Blastocyst / chemistry*
  • Blastocyst / ultrastructure
  • Cattle / embryology
  • DNA / analysis*
  • DNA / biosynthesis
  • RNA / analysis*
  • RNA / biosynthesis
  • Specimen Handling

Substances

  • RNA
  • DNA