Intergenic suppression of stage II sporulation defects by a mutation in the major vegetative sigma factor gene (rpoD) of Bacillus subtilis

Biochimie. 1992 Jul-Aug;74(7-8):635-40. doi: 10.1016/0300-9084(92)90135-2.

Abstract

The Bacillus subtilis intergenic suppressor mutations crsA and rvtA, previously shown to restore sporulation competence to a variety of strains containing stage 0 sporulation defects, also suppress lesions in the stage II sporulation genes spoIIF, spoIIN and spoIIJ. They do not rescue sporulation in other stage II through stage V sporulation mutations. Cells containing spoIIN, spoIIF96 and spoIIJ::Tn917 mutations fail to transcribe spoIID, a late stage II gene. Introduction of crsA47 into spoIINts279, spoIIF96, or spoIIJ::Tn917 mutant backgrounds circumvents the need for the spoIIF, IIN, and IIJ products, restoring both expression of spoIID, and sporulation competence.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacillus subtilis / genetics*
  • Bacillus subtilis / physiology
  • Bacterial Proteins / genetics*
  • DNA-Directed RNA Polymerases*
  • Electrophoresis, Polyacrylamide Gel
  • Gene Expression Regulation, Bacterial
  • Genotype
  • Mutation
  • Promoter Regions, Genetic
  • Sigma Factor / genetics*
  • Spores, Bacterial / physiology*
  • Suppression, Genetic*
  • Transcription, Genetic

Substances

  • Bacterial Proteins
  • Sigma Factor
  • RNA polymerase sigma 70
  • DNA-Directed RNA Polymerases