Purification and characterization of streptolysin O from Streptococcus pyogenes

Int J Biochem. 1992 Jul;24(7):1073-9. doi: 10.1016/0020-711x(92)90376-c.

Abstract

1. Streptolysin O, an exotoxin produced by group A beta-hemolytic streptococci, has been purified from Streptococcus pyogenes culture supernatants. 2. The isolation and purification procedure involved ammonium sulphate and polyethylene glycol precipitations, and ion-exchange chromatographies on CM-Sepharose and Mono Q. 3. The proposed procedure introduces two ion-exchange chromatography steps making the purification process simpler and, especially, more reproducible than other described protocols. 4. The purified streptolysin O was hemolytically active, had a specific activity of 415,000 HU/mg, an optimum pH of 7.0, a relative molecular mass of 60,100 and an isoelectric pH of 7.5.

MeSH terms

  • Bacterial Proteins
  • Electrophoresis, Polyacrylamide Gel
  • Erythrocyte Count
  • Hydrogen-Ion Concentration
  • Isoelectric Focusing
  • Reproducibility of Results
  • Streptococcus pyogenes / chemistry*
  • Streptolysins / antagonists & inhibitors
  • Streptolysins / chemistry
  • Streptolysins / isolation & purification*
  • Temperature
  • Tetanus Antitoxin

Substances

  • Bacterial Proteins
  • Streptolysins
  • Tetanus Antitoxin
  • streptolysin O