AKT2, a putative oncogene encoding a member of a subfamily of protein-serine/threonine kinases, is amplified in human ovarian carcinomas

Proc Natl Acad Sci U S A. 1992 Oct 1;89(19):9267-71. doi: 10.1073/pnas.89.19.9267.

Abstract

We isolated cDNA clones containing the entire coding region of the putative oncogene AKT2. Sequence analysis and in vitro translation demonstrated that AKT2 encodes a 56-kDa protein with homology to serine/threonine kinases; moreover, this protein contains a Src homology 2-like domain. AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. AKT2 was mapped to chromosome region 19q13.1-q13.2 by fluorescence in situ hybridization. In the two ovarian carcinoma cell lines exhibiting amplification of AKT2, the amplified sequences were localized within homogeneously staining regions. We conclude that AKT2 belongs to a distinct subfamily of protein-serine/threonine kinases containing Src homology 2-like domains and that alterations of AKT2 may contribute to the pathogenesis of ovarian carcinomas.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Chromosomes, Human / ultrastructure
  • Cloning, Molecular
  • DNA / genetics
  • DNA / isolation & purification
  • Female
  • Fluorescent Dyes
  • Gene Amplification
  • Gene Library
  • Humans
  • Lymphocytes / cytology
  • Molecular Sequence Data
  • Multigene Family*
  • Oncogene Proteins / genetics*
  • Oncogenes*
  • Ovarian Neoplasms / enzymology
  • Ovarian Neoplasms / genetics*
  • Protein Biosynthesis
  • Protein Serine-Threonine Kinases / genetics*
  • Proto-Oncogene Proteins c-akt
  • Proto-Oncogene Proteins*
  • Sequence Homology, Amino Acid
  • Transcription, Genetic

Substances

  • Fluorescent Dyes
  • Oncogene Proteins
  • Proto-Oncogene Proteins
  • DNA
  • AKT2 protein, human
  • Protein Serine-Threonine Kinases
  • Proto-Oncogene Proteins c-akt

Associated data

  • GENBANK/M95936