Abstract
Activin A, a homodimer of the beta A chain, regulates hematopoiesis. In a human bone marrow-derived stromal cell line, KM-102, phorbol myristate acetate, tumor necrosis factor-alpha and interleukin-1 beta induced great increases in beta A chain mRNA levels and production of activin A activities. The phorbol ester-induced beta A chain gene expression was inhibited by cycloheximide and down regulation of protein kinase C, whereas the cytokine-induced expression was little affected by these treatments. These results indicate that the inflammatory cytokines directly stimulate beta A chain gene expression via protein kinase C-independent pathways.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Activins
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Blotting, Northern
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Bone Marrow / physiology*
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Bone Marrow Cells
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Cell Line
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Culture Media, Conditioned
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Cycloheximide / pharmacology
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DNA / analysis
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DNA / genetics
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Gene Expression / drug effects
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Growth Substances / biosynthesis
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Growth Substances / genetics*
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Growth Substances / isolation & purification
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Humans
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Inhibins / biosynthesis
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Inhibins / genetics*
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Inhibins / isolation & purification
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Interleukin-1 / pharmacology*
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Macromolecular Substances
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Phorbol 12,13-Dibutyrate / pharmacology
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Protein Kinase C / metabolism
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RNA, Messenger / metabolism
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Recombinant Proteins / pharmacology
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Tetradecanoylphorbol Acetate / pharmacology
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Tumor Necrosis Factor-alpha / pharmacology*
Substances
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Culture Media, Conditioned
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Growth Substances
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Interleukin-1
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Macromolecular Substances
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RNA, Messenger
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Recombinant Proteins
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Tumor Necrosis Factor-alpha
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Activins
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Phorbol 12,13-Dibutyrate
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Inhibins
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DNA
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Cycloheximide
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Protein Kinase C
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Tetradecanoylphorbol Acetate