Atypical pyramidal cells in epileptic human cortex: CFLS and 3-D reconstructions

Neuroreport. 1992 Sep;3(9):765-8. doi: 10.1097/00001756-199209000-00011.

Abstract

Epileptic temporal cortices, removed from 3 patients with intractable partial epilepsy (IPE) during neurosurgery, were studied. Pyramidal neurons (40-50 per slice) in laminae III, V and white matter, were injected with lucifer yellow. Samples were examined in a confocal laser scanning microscope (Biorad 600) and individual cells scanned at 0.1-1 microns incremental levels. 2-D maximal linear projection was used for overview. Frames (50-60) of scanned neurons were transformed into 3-D volumes, using VoxelView software on a Silicone Graphics workstation and rotated. All samples contained neurons with duplicated apical dendrites, additional basal dendrites or were misplaced in a horizontal position in the white matter. The relation between these preliminary observations and the disease is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Epilepsies, Partial / pathology*
  • Fluorescent Dyes
  • Humans
  • Image Processing, Computer-Assisted*
  • Isoquinolines
  • Lasers*
  • Neurons / ultrastructure
  • Temporal Lobe / pathology*
  • Temporal Lobe / ultrastructure

Substances

  • Fluorescent Dyes
  • Isoquinolines
  • lucifer yellow