Abstract
Protein-tyrosine kinases (PTKs) play an integral role in T cell activation. Stimulation of the T cell antigen receptor (TCR) results in tyrosine phosphorylation of a number of cellular substrates. One of these is the TCR zeta chain, which can mediate the transduction of extracellular stimuli into cellular effector functions. We have recently identified a 70 kd tyrosine phosphoprotein (ZAP-70) that associates with zeta and undergoes tyrosine phosphorylation following TCR stimulation. Here we report the isolation of a cDNA clone encoding ZAP-70. ZAP-70 represents a novel PTK and is expressed in T and natural killer cells. Moreover, tyrosine phosphorylation and association of ZAP-70 with zeta require the presence of src family PTKs and provide a potential mechanism by which the src family PTKs and ZAP-70 may interact to mediate TCR signal transduction.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Blotting, Northern
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Blotting, Western
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Cloning, Molecular
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Humans
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Membrane Proteins / metabolism*
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Molecular Sequence Data
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Phosphorylation
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Protein-Tyrosine Kinases / chemistry
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Protein-Tyrosine Kinases / genetics*
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Protein-Tyrosine Kinases / metabolism
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Receptors, Antigen, T-Cell / metabolism*
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Restriction Mapping
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Sequence Homology, Amino Acid
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Signal Transduction / physiology
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Tumor Cells, Cultured
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Tyrosine / metabolism
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ZAP-70 Protein-Tyrosine Kinase
Substances
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Membrane Proteins
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Receptors, Antigen, T-Cell
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antigen T cell receptor, zeta chain
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Tyrosine
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Protein-Tyrosine Kinases
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ZAP-70 Protein-Tyrosine Kinase
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ZAP70 protein, human
Associated data
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GENBANK/L03369
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GENBANK/L05148
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GENBANK/S47136
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GENBANK/S47137
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GENBANK/S47164
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GENBANK/S47165
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GENBANK/S47166
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GENBANK/S47167
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GENBANK/S47168
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GENBANK/S72771