Abstract
The aim of the present study was to investigate whether the early modulation of the c-fos and c-myc oncogenes could give some orientation to the impact of immunomodulators on the monocyte-macrophage lineage. In order to work in a homogeneous system we used the P388D1 mouse macrophage cell-line which is considered as an almost mature macrophage. When P388D1 cells were stimulated by LPS, interferon-gamma or the association of both compounds, no direct correlation could be found between the modulation of DNA synthesis and the early expression of the c-fos and c-myc oncogenes. The positive regulation of Ia antigen expression seemed to correlate with the absence of induction of c-fos oncogene.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adjuvants, Immunologic / pharmacology*
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Animals
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DNA Replication / drug effects
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Endotoxins / pharmacology*
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Gene Expression Regulation, Neoplastic / drug effects*
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Genes, MHC Class II / drug effects*
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Genes, fos / drug effects*
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Genes, myc / drug effects*
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Interferon-gamma / pharmacology*
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Leukemia P388 / genetics
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Leukemia P388 / pathology*
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Lipopolysaccharides
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Macrophage Activation / drug effects*
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Mice
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Recombinant Proteins
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Tetradecanoylphorbol Acetate / pharmacology
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Tumor Cells, Cultured / drug effects
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Tumor Cells, Cultured / metabolism
Substances
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Adjuvants, Immunologic
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Endotoxins
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Lipopolysaccharides
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Recombinant Proteins
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endotoxin, Escherichia coli
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Interferon-gamma
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Tetradecanoylphorbol Acetate