Capture ELISA for IgM antibodies against Plasmodium falciparum glutamate rich protein

J Immunol Methods. 1992 Nov 5;155(2):207-13. doi: 10.1016/0022-1759(92)90287-4.

Abstract

This report describes a novel mu chain capture ELISA for the detection of IgM antibodies against a Plasmodium falciparum antigen. A fragment of the 220 kDa P. falciparum glutamate rich protein containing amino acid residues 489-1271 was expressed in E. coli as a recombinant chimeric beta-galactosidase fusion protein and used as antigen after purification and biotinylation. Specific IgM antibodies were found in 51% (39/77) of sera from adult Liberians immune to malaria. The binding of IgM antibodies was specific for the malaria portion of the fusion protein and no cross-reactivity was found in sera from patients with IgM antibodies due to other diseases. Inhibition studies with a fusion protein containing amino acid residues 816-1134 (GLURP816-1134) representing the carboxy-terminal repeat region suggested a different use of epitopes for IgM antibodies in different individuals.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Protozoan / analysis*
  • Antigens, Protozoan / immunology*
  • Enzyme-Linked Immunosorbent Assay / methods
  • Humans
  • Immunoglobulin M / analysis*
  • Malaria, Falciparum / immunology
  • Plasmodium falciparum / immunology*
  • Protozoan Proteins / immunology*

Substances

  • Antibodies, Protozoan
  • Antigens, Protozoan
  • Immunoglobulin M
  • Protozoan Proteins
  • glutamate-rich protein, Plasmodium