Abstract
A second DNA site-specific (restriction) endonuclease (R.CviAII) and its cognate adenine DNA methyltransferase (M.CviAII) were isolated from virus PBCV-1 infected Chlorella strain NC64A cells. R.CviAII, a heteroschizomer of the bacterial restriction endonuclease NlaIII, recognizes the sequence CATG, and does not cleave CmATG sequences. However, unlike NlaIII, which cleaves after the G and does not cleave either CmATG or mCATG sequences, CviAII cleaves between the C and A and is unaffected by mCATG methylation. The M.CviAII and R.CviAII genes were cloned and their DNA sequences were determined. These genes are tandemly arranged head-to-tail such that the TAA termination codon of the M.CviAII methyltransferase gene overlaps the ATG translational start site of R.CviAII endonuclease. R.CviAII is the first chlorella virus site-specific endonuclease gene to be cloned and sequenced.
Publication types
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Amino Acid Sequence
-
Base Sequence
-
Chlorella / enzymology*
-
Chlorella / genetics
-
Cloning, Molecular
-
Deoxyribonucleases, Type II Site-Specific / chemistry
-
Deoxyribonucleases, Type II Site-Specific / genetics*
-
Deoxyribonucleases, Type II Site-Specific / metabolism
-
Escherichia coli / genetics
-
Genes, Viral / genetics*
-
Genomic Library
-
Methyltransferases / chemistry
-
Methyltransferases / genetics*
-
Methyltransferases / metabolism
-
Molecular Sequence Data
-
Site-Specific DNA-Methyltransferase (Adenine-Specific) / chemistry
-
Site-Specific DNA-Methyltransferase (Adenine-Specific) / genetics*
-
Site-Specific DNA-Methyltransferase (Adenine-Specific) / metabolism
-
Viral Proteins / chemistry
-
Viral Proteins / genetics*
-
Viral Proteins / metabolism
-
Viral Structural Proteins / genetics*
-
Viruses / enzymology
-
Viruses / genetics
Substances
-
Viral Proteins
-
Viral Structural Proteins
-
DNA modification methylase PBCVI
-
Methyltransferases
-
Site-Specific DNA-Methyltransferase (Adenine-Specific)
-
endodeoxyribonuclease NlaIII
-
Deoxyribonucleases, Type II Site-Specific