Abstract
Both the envelope structural protein and the non-structural NS1 protein have been purified from the flavivirus dengue-2 by high-pressure liquid chromatography. These purified proteins maintain their reactivity with monoclonal antibodies. When tested in mice, the envelope protein elicited neutralizing antibodies and partially protected the animals against a lethal viral challenge. The mice responded to the non-structural protein by producing antibodies; however, these antibodies were not neutralizing and the mice were not protected.
MeSH terms
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Animals
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Antibodies, Viral / biosynthesis
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Blotting, Western
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Chromatography, High Pressure Liquid
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Chromatography, Ion Exchange
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Dengue / prevention & control*
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Dengue Virus / immunology*
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Electrophoresis, Polyacrylamide Gel
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Female
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Mice
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Mice, Inbred BALB C
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Neutralization Tests
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Radioimmunoassay
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Vaccines, Inactivated / immunology
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Viral Envelope Proteins / immunology*
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Viral Envelope Proteins / isolation & purification
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Viral Nonstructural Proteins / immunology*
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Viral Nonstructural Proteins / isolation & purification
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Viral Vaccines* / immunology
Substances
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Antibodies, Viral
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Vaccines, Inactivated
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Viral Envelope Proteins
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Viral Nonstructural Proteins
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Viral Vaccines