Purification of native dengue-2 viral proteins and the ability of purified proteins to protect mice

Am J Trop Med Hyg. 1992 Oct;47(4):405-12. doi: 10.4269/ajtmh.1992.47.405.

Abstract

Both the envelope structural protein and the non-structural NS1 protein have been purified from the flavivirus dengue-2 by high-pressure liquid chromatography. These purified proteins maintain their reactivity with monoclonal antibodies. When tested in mice, the envelope protein elicited neutralizing antibodies and partially protected the animals against a lethal viral challenge. The mice responded to the non-structural protein by producing antibodies; however, these antibodies were not neutralizing and the mice were not protected.

MeSH terms

  • Animals
  • Antibodies, Viral / biosynthesis
  • Blotting, Western
  • Chromatography, High Pressure Liquid
  • Chromatography, Ion Exchange
  • Dengue / prevention & control*
  • Dengue Virus / immunology*
  • Electrophoresis, Polyacrylamide Gel
  • Female
  • Mice
  • Mice, Inbred BALB C
  • Neutralization Tests
  • Radioimmunoassay
  • Vaccines, Inactivated / immunology
  • Viral Envelope Proteins / immunology*
  • Viral Envelope Proteins / isolation & purification
  • Viral Nonstructural Proteins / immunology*
  • Viral Nonstructural Proteins / isolation & purification
  • Viral Vaccines* / immunology

Substances

  • Antibodies, Viral
  • Vaccines, Inactivated
  • Viral Envelope Proteins
  • Viral Nonstructural Proteins
  • Viral Vaccines