Abstract
A nuclear glycoprotein with an apparent Mr of 66,000 Da has been isolated from pig kidney chromatin after extraction with urea, guanidine-HCl and 2 M NaCl, and some of its structural features have been characterized. It belongs to the group of N-glycosylated proteins, which in the nucleus has so far received little attention. From its monosaccharide composition and recognition by lectins its oligosaccharides appear to be of high mannose and/or hybrid types. Some properties of its protein moiety suggest that it has a role in the packing of the DNA loops in the condensed chromatin.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Amino Acids / analysis
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Animals
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Carbohydrate Sequence
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Carbohydrates / analysis
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Chromatin / chemistry
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Chromatography, High Pressure Liquid
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Electrophoresis, Gel, Two-Dimensional
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Glycoproteins / genetics
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Glycoproteins / isolation & purification*
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Glycoproteins / metabolism
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Kidney / chemistry*
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Lectins / metabolism
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Molecular Sequence Data
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Nuclear Proteins / genetics
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Nuclear Proteins / isolation & purification*
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Nuclear Proteins / metabolism
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Sequence Homology, Amino Acid
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Structure-Activity Relationship
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Swine
Substances
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Amino Acids
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Carbohydrates
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Chromatin
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Glycoproteins
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Lectins
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Nuclear Proteins