Abstract
A panel of CD4+ T-cell clones has been isolated from the spleen and lymph nodes of diabetic NOD mice. These clones have been shown to be islet-specific both in vivo and in vitro. One of the clones, BDC-6.9, initiates extensive damage to islet tissue when placed adjacent to an NOD islet graft that has been used to reverse diabetes in (CBA x NOD)F1 recipients or when injected intraperitoneally into such animals. In this study, we show that BDC-6.9 T cells can initiate islet destruction in the absence of detectable CD8 T cells either in the periphery or in the lesion that develops after the transfer of the cloned islet-reactive T cells.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibodies, Monoclonal / immunology
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Blood Glucose / metabolism
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CD4 Antigens / immunology*
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CD8 Antigens / immunology*
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Cells, Cultured
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Crosses, Genetic
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Diabetes Mellitus, Type 1 / blood
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Diabetes Mellitus, Type 1 / immunology*
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Diabetes Mellitus, Type 1 / surgery
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Female
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Flow Cytometry
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Islets of Langerhans / immunology*
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Islets of Langerhans Transplantation / immunology*
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Islets of Langerhans Transplantation / pathology
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Kidney Transplantation / immunology*
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Kidney Transplantation / pathology
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Lymph Nodes / immunology
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Lymphocyte Depletion
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Male
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Mice
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Mice, Inbred NOD
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Mice, Inbred Strains
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Spleen / immunology
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T-Lymphocyte Subsets / immunology*
Substances
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Antibodies, Monoclonal
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Blood Glucose
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CD4 Antigens
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CD8 Antigens