CD8 T cells are not required for islet destruction induced by a CD4+ islet-specific T-cell clone

Diabetes. 1992 Dec;41(12):1603-8. doi: 10.2337/diab.41.12.1603.

Abstract

A panel of CD4+ T-cell clones has been isolated from the spleen and lymph nodes of diabetic NOD mice. These clones have been shown to be islet-specific both in vivo and in vitro. One of the clones, BDC-6.9, initiates extensive damage to islet tissue when placed adjacent to an NOD islet graft that has been used to reverse diabetes in (CBA x NOD)F1 recipients or when injected intraperitoneally into such animals. In this study, we show that BDC-6.9 T cells can initiate islet destruction in the absence of detectable CD8 T cells either in the periphery or in the lesion that develops after the transfer of the cloned islet-reactive T cells.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Blood Glucose / metabolism
  • CD4 Antigens / immunology*
  • CD8 Antigens / immunology*
  • Cells, Cultured
  • Crosses, Genetic
  • Diabetes Mellitus, Type 1 / blood
  • Diabetes Mellitus, Type 1 / immunology*
  • Diabetes Mellitus, Type 1 / surgery
  • Female
  • Flow Cytometry
  • Islets of Langerhans / immunology*
  • Islets of Langerhans Transplantation / immunology*
  • Islets of Langerhans Transplantation / pathology
  • Kidney Transplantation / immunology*
  • Kidney Transplantation / pathology
  • Lymph Nodes / immunology
  • Lymphocyte Depletion
  • Male
  • Mice
  • Mice, Inbred NOD
  • Mice, Inbred Strains
  • Spleen / immunology
  • T-Lymphocyte Subsets / immunology*

Substances

  • Antibodies, Monoclonal
  • Blood Glucose
  • CD4 Antigens
  • CD8 Antigens