Abstract
Although T cell receptor (TCR)gammadelta+ and TCRalphabeta+ cells are commonly viewed as functionally independent, their relatedness and potential interdependence remain enigmatic. Here we have identified a gene profile that distinguishes mouse gammadelta cell populations from conventional alphabeta T cells. However, this profile was also expressed by sets of unconventional alphabeta T cells. Therefore, whereas TCR specificity determines the involvement of a T cell in an immune response, the cell's functional potential, as assessed by gene expression, does not segregate with the TCR. By monitoring the described gene profile, we show that gammadelta T cell development and function in TCRbeta-deficient mice was impaired because of the absence of alphabeta T cell progenitors. Thus, normal gammadelta cell development is dependent on the development of conventional alphabeta T cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Differentiation / genetics
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Cell Differentiation / immunology
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Cyclic AMP Response Element Modulator
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / immunology
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Flow Cytometry
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Gene Expression Profiling
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Genes, T-Cell Receptor / genetics*
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Genes, T-Cell Receptor / immunology
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H-Y Antigen / genetics
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H-Y Antigen / immunology
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Male
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Mice, Transgenic
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics
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Receptors, Antigen, T-Cell, alpha-beta / genetics*
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Receptors, Antigen, T-Cell, alpha-beta / immunology
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Receptors, Antigen, T-Cell, gamma-delta / genetics*
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Receptors, Antigen, T-Cell, gamma-delta / immunology
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Repressor Proteins*
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Reverse Transcriptase Polymerase Chain Reaction
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T-Lymphocytes / cytology
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T-Lymphocytes / immunology*
Substances
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DNA-Binding Proteins
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H-Y Antigen
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RNA, Messenger
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Receptors, Antigen, T-Cell, alpha-beta
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Receptors, Antigen, T-Cell, gamma-delta
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Repressor Proteins
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Cyclic AMP Response Element Modulator