Atrial cardiomyocyte-specific expression of Cre recombinase driven by an Nppa gene fragment

Genesis. 2003 Sep;37(1):1-4. doi: 10.1002/gene.10220.

Abstract

To study the development of the atria, we produced a transgenic mouse line that expresses Cre under the regulatory control of a 7 kbp fragment of the Natriuretic peptide precursor type A gene (Nppa), from -3 kbp to +4 kbp relative to the transcription start site. Crossing this line with the R26R and Z/EG reporter lines revealed recombinase activity specifically in the cardiomyocytes of the atria and to a lesser extent the inflow tract from E10.5 onwards. At E14.5 recombination in the atria is almost complete. No recombination was observed outside the heart. These mice provide a tool to study gene function in the atria.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Atrial Natriuretic Factor
  • Cell Line
  • Genes, Reporter
  • Heart Atria / cytology*
  • Heart Atria / embryology
  • Heterozygote
  • Integrases / metabolism*
  • Mice
  • Mice, Transgenic
  • Microscopy, Fluorescence
  • Models, Genetic
  • Myocytes, Cardiac / enzymology*
  • Natriuretic Agents / chemistry
  • Natriuretic Peptide, C-Type / genetics*
  • Protein Precursors / genetics*
  • Recombination, Genetic
  • Time Factors
  • Viral Proteins / metabolism*
  • beta-Galactosidase / metabolism

Substances

  • Natriuretic Agents
  • Nppa protein, mouse
  • Protein Precursors
  • Viral Proteins
  • Natriuretic Peptide, C-Type
  • Atrial Natriuretic Factor
  • Cre recombinase
  • Integrases
  • beta-Galactosidase