Effects of lipid-based oral formulations on plasma and tissue amphotericin B concentrations and renal toxicity in male rats

Antimicrob Agents Chemother. 2003 Oct;47(10):3339-42. doi: 10.1128/AAC.47.10.3339-3342.2003.

Abstract

The purpose of this study was to determine the effects of various lipid and mixed-micelle formulations on the oral absorption and renal toxicity of amphotericin B (AMB) in rats. The maximum concentration of AMB in plasma and the area under the concentration-time curve for 0 to 24 h for AMB were elevated in rats administered triglyceride (TG)-rich AMB formulations in comparison to those in rats given (i) AMB preformulated as a micelle containing sodium deoxycholate with sodium phosphate as a buffer (DOC-AMB), (ii) an AMB-lipid complex suspension, or (iii) AMB solubilized in methanol. Furthermore, our findings suggest that AMB incorporated into TG-based oral formulations has less renal toxicity than DOC-AMB.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Oral
  • Amphotericin B / blood
  • Amphotericin B / chemistry
  • Amphotericin B / pharmacokinetics*
  • Amphotericin B / toxicity*
  • Animals
  • Antifungal Agents / blood
  • Antifungal Agents / chemistry
  • Antifungal Agents / pharmacokinetics
  • Antifungal Agents / toxicity
  • Area Under Curve
  • Creatinine / blood
  • Deoxycholic Acid / chemistry
  • Deoxycholic Acid / pharmacokinetics
  • Drug Delivery Systems
  • Kidney Diseases / chemically induced*
  • Lipids / chemistry
  • Lipids / pharmacokinetics*
  • Male
  • Micelles
  • Phosphates / chemistry
  • Phosphates / pharmacokinetics
  • Rats
  • Rats, Sprague-Dawley
  • Tissue Distribution
  • Triglycerides / chemistry
  • Triglycerides / pharmacokinetics

Substances

  • Antifungal Agents
  • Lipids
  • Micelles
  • Phosphates
  • Triglycerides
  • Deoxycholic Acid
  • Amphotericin B
  • Creatinine
  • sodium phosphate