Insertion of the CCND1 gene into the IgH locus in a case of leukaemic small cell mantle lymphoma with normal chromosomes 11 and 14

J Clin Pathol. 2003 Oct;56(10):798-800. doi: 10.1136/jcp.56.10.798.

Abstract

The t(11;14)(q13;q32) translocation is considered to be the cytogenetic hallmark of mantle cell lymphoma. This report describes a case of leukaemic mantle cell lymphoma in which conventional cytogenetics on stimulated peripheral blood cells showed a 46,XY, t(1;12)(p21;q23)/46,XY karyotype. Fluorescence in situ hybridisation analysis using a dual colour immunoglobulin heavy chain (IgH)/CCND1 probe showed a fusion hybridisation signal on one normal chromosome 14, indicating that an insertion of the CCND1 gene into the 14q32/IgH locus had taken place. Overexpression of the cyclin D1 protein was demonstrated on bone marrow trephine by immunohistochemical staining.

Publication types

  • Case Reports

MeSH terms

  • Biomarkers, Tumor / analysis
  • Bone Marrow Cells / chemistry
  • Bone Marrow Cells / pathology
  • Chromosomes, Human, Pair 11
  • Chromosomes, Human, Pair 12
  • Cyclin D1 / analysis
  • Cyclin D1 / genetics*
  • Cytogenetic Analysis
  • Humans
  • Immunoglobulin Heavy Chains / genetics*
  • Immunohistochemistry
  • In Situ Hybridization, Fluorescence
  • Leukemic Infiltration*
  • Lymphoma, Mantle-Cell / genetics*
  • Lymphoma, Mantle-Cell / immunology
  • Male
  • Middle Aged
  • Mutagenesis, Insertional*

Substances

  • Biomarkers, Tumor
  • Immunoglobulin Heavy Chains
  • Cyclin D1