Abstract
The human dihydrofolate reductase and mismatch repair protein 1 genes are organized in a head-to-head configuration separated by an 88 base-pair segment and directed by a bidirectional promoter. In vivo transient assays of the site directed mutant promoters using firefly luciferase as a reporter showed that an AT-rich sequence, ACAAATA, in the GC-rich promoter sequence is not required for transcription. However, two out of four GC boxes were shown to function as bidirectional positive regulatory elements. Among them, a GC box at the midpoint of the region between the two initiation sites is essential for supporting minimal bidirectional activity.
MeSH terms
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Adenosine Triphosphatases*
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Bacterial Proteins / genetics
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Base Sequence
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Consensus Sequence
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DNA Mutational Analysis
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DNA-Binding Proteins*
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Escherichia coli Proteins*
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Humans
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Luciferases / biosynthesis
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Luciferases / genetics
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Molecular Sequence Data
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Multidrug Resistance-Associated Proteins*
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MutS DNA Mismatch-Binding Protein
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MutS Homolog 3 Protein
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Promoter Regions, Genetic / genetics*
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Protein Biosynthesis
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Proteins / genetics*
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / genetics
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Structure-Activity Relationship
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TATA Box
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Tetrahydrofolate Dehydrogenase / genetics*
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Transcription, Genetic*
Substances
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Bacterial Proteins
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DNA-Binding Proteins
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Escherichia coli Proteins
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MSH3 protein, human
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Multidrug Resistance-Associated Proteins
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MutS Homolog 3 Protein
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Proteins
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Recombinant Fusion Proteins
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Luciferases
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Tetrahydrofolate Dehydrogenase
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Adenosine Triphosphatases
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MutS DNA Mismatch-Binding Protein
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MutS protein, E coli
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multidrug resistance-associated protein 1