Abstract
Nardostachin, which is an iridoid isolated from Patrinia saniculaefolia, was examined by assessing its effect on the production of tumor necrosis factor-alpha (TNF-alpha) and expression of 2 enzymes, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), in lipopolysaccharide (LPS)-stimulated Raw264.7 macrophages. This compound consistently inhibited the production of nitric oxide (NO) and TNF-alpha production in a dose-dependent manner, with respective IC(50) values of 12.3 and 16.2 microM. The decrease in quantity of NO products was accompanied by a decrease in the iNOS protein level, as assessed by Western blotting probed with specific anti-iNOS antibodies. In addition, this compound also reduced the COX-2 protein expression level and the attendant PGE(2) production in LPS-stimulated macrophages. These results suggest that nardostachin may be useful for inhibiting the production of inflammatory mediators such as TNF-alpha, NO and PGE(2) in inflammatory diseases.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cyclooxygenase 2
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Dinoprostone / antagonists & inhibitors*
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Dinoprostone / metabolism
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Diterpenes / pharmacology*
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Dose-Response Relationship, Drug
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Isoenzymes / antagonists & inhibitors
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Isoenzymes / biosynthesis
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Lipopolysaccharides / pharmacology
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Macrophages / drug effects
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Macrophages / metabolism*
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Mice
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Nitric Oxide / antagonists & inhibitors*
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Nitric Oxide / biosynthesis
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Nitric Oxide Synthase / antagonists & inhibitors
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Nitric Oxide Synthase / biosynthesis
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Nitric Oxide Synthase Type II
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Patrinia
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Plant Extracts / isolation & purification
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Plant Extracts / pharmacology
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Plant Structures
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Prostaglandin-Endoperoxide Synthases / biosynthesis
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Tumor Necrosis Factor-alpha / antagonists & inhibitors*
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Tumor Necrosis Factor-alpha / biosynthesis*
Substances
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Diterpenes
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Isoenzymes
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Lipopolysaccharides
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Plant Extracts
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Tumor Necrosis Factor-alpha
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nardostachin
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Nitric Oxide
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Nitric Oxide Synthase
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Nitric Oxide Synthase Type II
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Nos2 protein, mouse
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Cyclooxygenase 2
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Prostaglandin-Endoperoxide Synthases
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Dinoprostone