Abstract
Proteasomes are intracellular protein complexes displaying multiproteolytic activities. These complexes have been implicated in the antigen degradation process that generates peptides associated with the major histocompatibility complex (MHC) class-I molecule. RING10 and RING12 are genes encoded by the class-II region of the human MHC that have sequence homology to proteasome-encoding genes. We have identified a yeast gene, called PRG1, that encodes a protein predicted to contain 55.6% sequence identity to 80% of the RING10 gene product. Genomic disruption of PRG1 revealed that it is essential for yeast cell growth. These data strongly indicate that the antigen-processing system present in vertebrates evolved from a basic cellular process present in all organisms.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cysteine Endopeptidases / metabolism*
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Fungal Proteins / genetics*
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Humans
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Molecular Sequence Data
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Multienzyme Complexes / metabolism*
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Proteasome Endopeptidase Complex
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Proteins / genetics*
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Restriction Mapping
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / growth & development
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Saccharomyces cerevisiae Proteins*
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Sequence Homology, Amino Acid
Substances
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Fungal Proteins
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Multienzyme Complexes
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Proteins
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Saccharomyces cerevisiae Proteins
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Cysteine Endopeptidases
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LMP7 protein
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PRE2 protein, S cerevisiae
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Proteasome Endopeptidase Complex
Associated data
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GENBANK/M79310
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GENBANK/M81114
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GENBANK/M81115
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GENBANK/M81116
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GENBANK/M81117
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GENBANK/M81118
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GENBANK/M95864
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GENBANK/M96667
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GENBANK/X65112
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GENBANK/X65113