Abstract
Vaccination with autologous leukaemia-derived dendritic cells (DC) presents an adjuvant treatment option for chronic myeloid leukaemia (CML). Here, we show that high-efficiency CD40-targeted adenoviral gene transfer of GM-CSF to CML-derived DC induces long-lived maturation in the absence of exogenous cytokines and may thus ensure protracted stimulation of CML-specific T cells upon vaccination.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenoviridae / genetics*
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CD40 Antigens / genetics*
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CD40 Antigens / metabolism
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Cytokines / metabolism
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Dendritic Cells / immunology*
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Gene Transfer Techniques
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Genetic Vectors
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Granulocyte-Macrophage Colony-Stimulating Factor / genetics*
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Granulocyte-Macrophage Colony-Stimulating Factor / metabolism
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Humans
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Immunophenotyping
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive / immunology
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive / therapy*
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T-Lymphocytes, Cytotoxic / immunology
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T-Lymphocytes, Cytotoxic / metabolism
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Transduction, Genetic
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Tumor Cells, Cultured
Substances
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CD40 Antigens
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Cytokines
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Granulocyte-Macrophage Colony-Stimulating Factor