Abstract
Recent data in yeast and Drosophila suggest a domain-like centromere structure with a modified chromatin core and flanking regions of heterochromatin. We have analyzed a functional human centromere and defined a region of increased chromosome scaffold/matrix attachment that overlaps three other distinct and nonoverlapping domains for constitutive centromere proteins CENP-A and CENP-H, and heterochromatin protein HP1. Transcriptional competency is intact throughout the S/MAR-enriched region and within the CENP-A- and CENP-H-associated chromatin. These results provide insights into the relationship between centromeric chromatin and transcriptional competency in vivo, highlighting the permissibility of transcription within the constitutively modified, nonheterochromatic chromatin of a functional eukaryotic centromere.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Autoantigens*
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Centromere / metabolism*
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Centromere / ultrastructure*
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Centromere Protein A
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Chromatin / metabolism
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Chromosomal Proteins, Non-Histone / chemistry
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Chromosomes, Artificial, Bacterial
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Chromosomes, Human, Pair 10 / ultrastructure
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DNA-Binding Proteins*
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Drosophila
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Gene Expression Regulation
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Hepatocyte Nuclear Factor 1
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Hepatocyte Nuclear Factor 1-alpha
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Hepatocyte Nuclear Factor 1-beta
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Heterochromatin / metabolism
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Humans
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Hybrid Cells / metabolism
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Models, Genetic
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Nuclear Proteins*
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Protein Binding
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Protein Structure, Tertiary
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Transcription Factors / metabolism
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Transcription, Genetic*
Substances
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Autoantigens
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CENPA protein, human
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CENPH protein, human
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Centromere Protein A
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Chromatin
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Chromosomal Proteins, Non-Histone
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DNA-Binding Proteins
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HNF1A protein, human
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HNF1B protein, human
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Hepatocyte Nuclear Factor 1-alpha
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Heterochromatin
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Nuclear Proteins
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Transcription Factors
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Hepatocyte Nuclear Factor 1
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Hepatocyte Nuclear Factor 1-beta