Abstract
ARO4 and HIS7 are two tandemly orientated genes of Saccharomyces cerevisiae that are transcribed into the same direction. The ARO4 terminator and the HIS7 promoter regions are sensitive to Micrococcus nuclease (Mnase) and separated by a positioned nucleosome. The HIS7 promoter is target for the transcription factors Gcn4p and Bas1p/Bas2p that activate its transcription upon amino acid starvation and purine limitation, respectively. Activation of the HIS7 gene by Gcn4p overexpression but not by Bas1p/Bas2p releases an ordered nucleosome distribution to yield increased Mnase sensitivity throughout the intergenic region. This remodeling is SNF2 dependent but mostly GCN5 independent. Accordingly, SNF2 is necessary for the Gcn4p-mediated transcriptional activation of the HIS7 gene. GCN5 is required for activation upon adenine limitation by Bas1p/Bas2p. Our data suggest that activation of HIS7 transcription by Gcn4p and Bas1p/Bas2p is supported by a nucleosome position-dependent and -independent mechanism, respectively. Whereas Gcn4p activation causes Swi/Snf-mediated remodeling of the nucleosomal architecture at the HIS7 promoter, the Bas1p/Bas2p complex presumably activates in combination with Gcn5p-dependent histone acetylation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3-Deoxy-7-Phosphoheptulonate Synthase
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Adenine / metabolism
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Adenosine Triphosphatases
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Aldehyde-Lyases / genetics
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Aminohydrolases
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Blotting, Northern
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Blotting, Southern
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Chromatin / chemistry
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Chromatin / genetics
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Chromatin / metabolism
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DNA, Intergenic / genetics
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / physiology
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Deoxyribonucleases / metabolism
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Gene Expression Regulation, Fungal
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Genotype
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Histone Acetyltransferases
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Homeodomain Proteins / genetics
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Homeodomain Proteins / physiology
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Models, Genetic
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Multienzyme Complexes / genetics*
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Nuclear Proteins*
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Nucleosomes / genetics*
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Nucleosomes / physiology
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Plasmids / genetics
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Promoter Regions, Genetic / genetics
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Protein Kinases / genetics
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Protein Kinases / physiology
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / physiology
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / physiology
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Trans-Activators / genetics
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Trans-Activators / physiology
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Transcription Factors / genetics
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Transcription Factors / physiology
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Transcriptional Activation
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Transferases / genetics*
Substances
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BAS1 protein, S cerevisiae
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Chromatin
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DNA, Intergenic
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DNA-Binding Proteins
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Homeodomain Proteins
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Multienzyme Complexes
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Nuclear Proteins
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Nucleosomes
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PHO2 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Trans-Activators
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Transcription Factors
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Transferases
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GCN5 protein, S cerevisiae
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Histone Acetyltransferases
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3-Deoxy-7-Phosphoheptulonate Synthase
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Protein Kinases
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Deoxyribonucleases
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imidazole glycerol phosphate synthase
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Aminohydrolases
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Adenosine Triphosphatases
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SNF2 protein, S cerevisiae
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Aldehyde-Lyases
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Adenine