Abstract
Five known kaurane type diterpenoids, 16alphaH,17-isovaleryloxy-ent-kauran-19-oic acid (1), 16alpha-hydroxy-17-isovaleryloxy-ent-kauran-19-oic acid (2), paniculoside-IV (3), 16alpha-hydroxy-ent-kauran-19-oic acid (4), and ent-kaur-16-en-19-oic acid (5) were isolated from the root of Acanthopanax koreanum by repeated column chromatography and reversed phase preparative HPLC. The structures of these compounds were established from physicochemical and spectral data. Among the isolated compounds 16alphaH,17-isovaleryloxy-ent-kauran-19-oic acid (1) showed potent inhibitory activity (IC50 value, 16.2 uM) on TNF-alpha secretion from HMC-1, a trypsin-stimulated human leukemic mast cell line.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Diterpenes, Kaurane / chemistry*
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Diterpenes, Kaurane / isolation & purification
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Diterpenes, Kaurane / pharmacology*
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Eleutherococcus / chemistry*
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Flavonoids / pharmacology
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Humans
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Korea
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Leukemia, Mast-Cell / chemically induced
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Leukemia, Mast-Cell / metabolism
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Luteolin
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Methanol
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Plant Extracts / chemistry
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Plant Roots / chemistry
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Plants, Medicinal / chemistry
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Trypsin / pharmacology*
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Tumor Cells, Cultured
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Tumor Necrosis Factor-alpha / antagonists & inhibitors*
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Tumor Necrosis Factor-alpha / metabolism*
Substances
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16alpha-hydroxy-17-isovaleryloxy-ent-kauran-19-oic acid
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Diterpenes, Kaurane
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Flavonoids
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Plant Extracts
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Tumor Necrosis Factor-alpha
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Trypsin
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Luteolin
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Methanol