Abstract
Nonradioactive techniques have been used for the direct detection of hepatitis B virus DNA in human serum samples. A comparison of two different systems using digoxigenin-labeled DNA probes is presented. Furthermore, oligonucleotides containing one molecule of the hapten digoxigenin at the 5'-end were prepared and used as primers for the polymerase chain reaction. Amplified DNA can be directly analyzed with anti-digoxigenin Fab fragments labeled with alkaline phosphatase and chemiluminescent substrates.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkaline Phosphatase
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Base Sequence
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DNA Probes* / chemical synthesis
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DNA, Viral / blood*
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Digoxigenin*
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Gene Amplification
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Hepatitis B / blood
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Hepatitis B virus / genetics*
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Humans
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Immunoglobulin Fragments
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Luminescent Measurements
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Molecular Sequence Data
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Oligonucleotide Probes*
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Oligonucleotides / chemical synthesis
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Polymerase Chain Reaction / methods*
Substances
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DNA Probes
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DNA, Viral
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Immunoglobulin Fragments
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Oligonucleotide Probes
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Oligonucleotides
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Alkaline Phosphatase
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Digoxigenin