MALDI-TOF mass spectrometry as a powerful tool to study enzymatic processing of DNA lesions inserted into oligonucleotides

D Gasparutto; C Saint-Pierre; M Jaquinod; A Favier; J Cadet

doi:10.1081/NCN-120023039

MALDI-TOF mass spectrometry as a powerful tool to study enzymatic processing of DNA lesions inserted into oligonucleotides

Nucleosides Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):1583-6. doi: 10.1081/NCN-120023039.

Authors

D Gasparutto¹, C Saint-Pierre, M Jaquinod, A Favier, J Cadet

Affiliation

¹ Laboratoire des Lésions des Acides Nucléiques, Service Chimie Inorganique Biologique-UMR 5046, DRFMC, CEA-Grenoble, Grenoble Cedex, France. [email protected]

PMID: 14565471
DOI: 10.1081/NCN-120023039

Abstract

MALDI-TOF mass spectrometry measurements, coupled with either exonuclease or DNA N-glycosylases digestions of lesion-containing oligonucleotides, were used to assess biochemical features of several oxidative DNA damage. The latter analytical approach was shown to be an informative and efficient alternative technique to conventional electrophoresis and chromatographic analyses.

MeSH terms

Animals
Base Pair Mismatch / genetics
Cattle
DNA / chemistry*
DNA Damage*
DNA Glycosylases
Deoxyribonucleases
Oligodeoxyribonucleotides / chemistry*
Phosphodiesterase I
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods

Substances

Oligodeoxyribonucleotides
DNA
Deoxyribonucleases
Phosphodiesterase I
phosphodiesterase I, snake venom
DNA Glycosylases