Cell surface oligonucleotide-binding proteins of human squamous carcinoma A431 cells

P P Laktionov; B P Chelobanov; M V Kharkova; E Yu Rykova; D V Pyshnyi; I A Pyshnaya; K Marcus; H E Meyer; V V Vlassov

doi:10.1081/NCN-120023121

Cell surface oligonucleotide-binding proteins of human squamous carcinoma A431 cells

Nucleosides Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):1715-9. doi: 10.1081/NCN-120023121.

Authors

P P Laktionov¹, B P Chelobanov, M V Kharkova, E Yu Rykova, D V Pyshnyi, I A Pyshnaya, K Marcus, H E Meyer, V V Vlassov

Affiliation

¹ Group of Cell Biology, Novosibirsk Institute of Bioorganic Chemistry SB RAS, Novosibirsk, Russia. lakt.niboch.nsc

PMID: 14565503
DOI: 10.1081/NCN-120023121

Abstract

Affinity modified with Flu-DAP-p(N)16degU oligonucleotide-binding proteins were isolated by affinity chromatography using Ultrogel A2-anti fluorescein antibodies. After separation by SDS-PAGE the proteins with molecular masses about 68 kDa were MS/MS sequenced and identified as keratin K1, keratin K10, keratin K2e and albumin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Binding Sites
Carcinoma, Squamous Cell
Chromatography, Affinity
Chromatography, Gel
Humans
Keratin-10
Keratin-2
Keratins / chemistry
Keratins / isolation & purification
Keratins / metabolism
Membrane Proteins / chemistry
Membrane Proteins / isolation & purification
Membrane Proteins / metabolism*
Molecular Weight
Neoplasm Proteins / chemistry
Neoplasm Proteins / isolation & purification
Neoplasm Proteins / metabolism
Oligonucleotides / metabolism*
Protein Binding
Serum Albumin / isolation & purification
Serum Albumin / metabolism
Tumor Cells, Cultured

Substances

KRT10 protein, human
KRT2 protein, human
Keratin-2
Membrane Proteins
Neoplasm Proteins
Oligonucleotides
Serum Albumin
Keratin-10
Keratins