Abstract
Nine new epothilone analogues (6-8, 10, 11a, 11b, 12, 13, and 15) were isolated from fermentations of Myxococcus xanthus strains engineered with modified polyketide synthase genes. The epothilone structures were elucidated primarily through interpretation of 1D and 2D NMR data. 4-Desmethyl-10,11-didehydroepothilone D (6) displayed activity against several tumor cell lines, including a multi-drug-resistant cell line.
MeSH terms
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Antineoplastic Agents / chemistry
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Antineoplastic Agents / isolation & purification*
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Antineoplastic Agents / pharmacology
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Cell Line, Tumor / drug effects
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Drug Resistance, Multiple / drug effects
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Drug Screening Assays, Antitumor
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Epothilones / chemistry
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Epothilones / isolation & purification*
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Epothilones / pharmacology
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Fermentation
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Humans
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Inhibitory Concentration 50
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Molecular Structure
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Myxococcus xanthus / chemistry*
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Nuclear Magnetic Resonance, Biomolecular
Substances
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Antineoplastic Agents
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Epothilones