Protection of swine against post-weaning multisystemic wasting syndrome (PMWS) by porcine circovirus type 2 (PCV2) proteins

Vaccine. 2003 Nov 7;21(31):4565-75. doi: 10.1016/s0264-410x(03)00503-6.

Abstract

Porcine circovirus type 2 (PCV2) is known to be associated with post-weaning multisystemic wasting syndrome (PMWS), a recently described disease of young pigs. Since no PCV2 vaccine was available so far, we have developed a specific PCV2 vaccine candidate. The Orf1-encoded replication protein and Orf2-encoded capsid protein of PCV2 were expressed and detected in either mammalian or insect expression systems. In a first trial, Orf2 protein was found to be a major immunogen, inducing protection in a prime-boost protocol; the piglets received a first injection with plasmids directing Orf2 protein and granulocyte-macrophage colony-stimulating factor (GM-CSF) expression, followed by a second injection, a fortnight later, associated with baculovirus-expressed Orf2 protein. As evaluated by growth parameters, clinical signs (fever), seroconversion, the pigs were protected against a PCV2 challenge after vaccination. In a second trial, protection induced by a subunit vaccine was even better than the one induced by DNA vaccine, since PCV2 replication was completely inhibited.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / analysis
  • Antibodies, Viral / biosynthesis
  • Circoviridae Infections / physiopathology
  • Circoviridae Infections / prevention & control*
  • Circoviridae Infections / veterinary*
  • Circovirus / immunology*
  • Cloning, Molecular
  • DNA, Viral / analysis
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Fever / physiopathology
  • Fever / prevention & control
  • Immunization, Secondary
  • Male
  • Reverse Transcriptase Polymerase Chain Reaction
  • Swine
  • Swine Diseases / physiopathology
  • Swine Diseases / prevention & control*
  • Vaccines, DNA / immunology
  • Viral Proteins / immunology*
  • Viral Vaccines / immunology*
  • Weight Gain / physiology

Substances

  • Antibodies, Viral
  • DNA, Viral
  • Vaccines, DNA
  • Viral Proteins
  • Viral Vaccines