Rapid detection and estimation by pyrosequencing of 23S rRNA genes with a single nucleotide polymorphism conferring linezolid resistance in Enterococci

Antimicrob Agents Chemother. 2003 Nov;47(11):3620-2. doi: 10.1128/AAC.47.11.3620-3622.2003.

Abstract

Pyrosequencing was used to detect rapidly and estimate the number of 23S rRNA genes with a G2576T mutation in 43 linezolid-resistant and -susceptible clinical isolates of enterococci. The method showed 100% concordance with PCR-restriction fragment length polymorphism for detecting isolates homozygous for either G2576 or T2576 or heterozygous for this mutation. A good correlation was found between linezolid MICs and the number of 23S rRNA gene copies carrying the mutation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetamides / pharmacology*
  • Anti-Infective Agents / pharmacology*
  • Drug Resistance, Bacterial
  • Enterococcus / drug effects*
  • Genes, Bacterial / genetics
  • Linezolid
  • Mutation / genetics
  • Oxazolidinones / pharmacology*
  • Polymorphism, Single Nucleotide / genetics*
  • RNA, Ribosomal, 23S / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Acetamides
  • Anti-Infective Agents
  • Oxazolidinones
  • RNA, Ribosomal, 23S
  • Linezolid