Anti malondialdehyde-adduct immunological response as a possible marker of successful aging

Exp Gerontol. 2003 Oct;38(10):1129-35. doi: 10.1016/s0531-5565(03)00188-8.

Abstract

Contrasting results have been obtained by various researchers about oxidative markers of aging. In this study, a healthy over-90-year-old population was examined for various plasma oxidative biomarkers and compared with a healthy population of blood donors (age range 23-66). Plasma malondialdehyde (MDA), evaluated by means of the thiobarbituric acid test, was significantly higher in the over-90-year-old population, confirming the presence of increased lipoperoxidation in old age. The antibody titre against MDA-protein adducts, considered a marker of lipoperoxidative protein damage in vivo, was evaluated in an ELISA test, completely home made and calibrated versus a concentrated pool of human plasma; this antibody titre was significantly higher in the over-90-year-old population. Plasma vitamin E, evaluated in RP-HPLC, was not significantly different between the two groups. Plasma protein-bound carbonyls, a marker of oxidative protein damage, were measured with the 2,4-dinitrophenylhydrazine assay; their level in the over-90-year-old population was lower than in the blood donors. The higher antibody titre against MDA-adducts may result in protection against accumulation of oxidatively damaged proteins by enhancing their removal, and, together with the preserved plasma vitamin E level, it may endow over-90-year-olds with an especially efficient antioxidant profile. The low level of protein carbonyl might reflect the more efficient removal of damaged proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Aging / blood
  • Aging / immunology*
  • Autoantibodies / blood*
  • Biomarkers / blood
  • Blood Proteins / analysis
  • Enzyme-Linked Immunosorbent Assay / methods
  • Humans
  • Lipid Peroxidation / physiology
  • Malondialdehyde / blood
  • Malondialdehyde / immunology*
  • Middle Aged
  • ROC Curve
  • Vitamin E / blood

Substances

  • Autoantibodies
  • Biomarkers
  • Blood Proteins
  • Vitamin E
  • Malondialdehyde