Abstract
It is well known that many cell types react strongly to micro-topography. It is rapidly becoming clear than cells will also react to nano-topography. Polymer demixing is a rapid and low-cost chemical method of producing nano-topography. This manuscript investigates human fibroblast response to 27nm high nano-islands produced by polymer demixing. Cell spreading, cytoskeleton, focal adhesion and Rac localisation were studied. The results showed that an initial rapid adhesion and cytoskeletal formation on the islands at 4 days of culture gave way to poorly formed contacts and vimentin cytoskeleton at 30 days of culture.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Biocompatible Materials / chemical synthesis
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Biocompatible Materials / chemistry*
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Cell Adhesion / physiology
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Cell Movement / physiology
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Cells, Cultured
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Complex Mixtures / chemistry
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Crystallization / methods
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Culture Techniques / instrumentation
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Culture Techniques / methods*
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Cytoskeleton / physiology
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Cytoskeleton / ultrastructure
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Extracellular Matrix / physiology
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Fibroblasts / cytology*
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Fibroblasts / physiology*
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Humans
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Materials Testing
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Membranes, Artificial
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Molecular Conformation
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Nanotechnology / instrumentation
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Nanotechnology / methods*
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Polymers / chemical synthesis
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Polymers / chemistry
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Polystyrenes / chemistry*
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Styrenes / chemistry*
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Surface Properties
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Tissue Engineering / instrumentation
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Tissue Engineering / methods*
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rac GTP-Binding Proteins / metabolism
Substances
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Biocompatible Materials
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Complex Mixtures
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Membranes, Artificial
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Polymers
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Polystyrenes
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Styrenes
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beta-bromostyrene
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rac GTP-Binding Proteins