Water-protein interactions in microcrystalline crh measured by 1H-13C solid-state NMR spectroscopy

J Am Chem Soc. 2003 Nov 5;125(44):13336-7. doi: 10.1021/ja036720y.

Abstract

Using solid-state NMR carbon-proton dipolar correlation spectroscopy, we observed hydrogen exchange on the millisecond time scale between water molecules and protein protons in a solid sample. These interactions are shown to be related to important structural features of the protein such as hydrogen-bonding or salt-bridge networks.

MeSH terms

  • Carbon Isotopes
  • Hydrogen Bonding
  • Nuclear Magnetic Resonance, Biomolecular / methods
  • Phosphoproteins / chemistry*
  • Protons
  • Water / chemistry*

Substances

  • Carbon Isotopes
  • Phosphoproteins
  • Protons
  • Water