Multiphoton multifocal microscopy exploiting a diffractive optical element

Opt Lett. 2003 Oct 15;28(20):1918-20. doi: 10.1364/ol.28.001918.

Abstract

Multiphoton multifocal microscopy (MMM) usually has been achieved through a combination of galvo scanners with microlens arrays, with rotating disks of microlens arrays, and cascaded beam splitters with asynchronous rastering of scanning mirrors. Here we describe the achievement of a neat and compact MMM by use of a high-diffraction-efficiency diffractive-optic element that generates a multiple-spot grid of uniform intensity to achieve higher fidelity in imaging of live cells at adequate speeds.