Impaired long-term potentiation in vivo in the dentate gyrus of pituitary adenylate cyclase-activating polypeptide (PACAP) or PACAP type 1 receptor-mutant mice

Shogo Matsuyama; Akira Matsumoto; Hitoshi Hashimoto; Norihito Shintani; Akemichi Baba

doi:10.1097/00001756-200311140-00017

Impaired long-term potentiation in vivo in the dentate gyrus of pituitary adenylate cyclase-activating polypeptide (PACAP) or PACAP type 1 receptor-mutant mice

Neuroreport. 2003 Nov 14;14(16):2095-8. doi: 10.1097/00001756-200311140-00017.

Authors

Shogo Matsuyama¹, Akira Matsumoto, Hitoshi Hashimoto, Norihito Shintani, Akemichi Baba

Affiliation

¹ Division of Molecular Pharmacology and Pharmacogenomics, Department of Genome Sciences, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan. [email protected]

PMID: 14600504
DOI: 10.1097/00001756-200311140-00017

Abstract

The present study was conducted to clarify a role of pituitary adenylate cyclase-activating polypeptide (PACAP) and PACAP type 1 receptor (PAC1R) in learning and memory function. We demonstrated long-term potentiation (LTP) in vivo in the dentate gyrus of PAC1R exon 2-deficient (PAC1R-/-) mice and heterozygous PACAP-deficient (PACAP+/-) mice using extracellular recording techniques. We used two paradigms of tetanic stimulation, suprathreshold and at threshold tetanus, which both induced LTP in vivo in PAC1R-/- and PACAP+/- mice. However, the population spike of 'at threshold' but not 'suprathreshold' LTP decreased significantly in PAC1R-/- and PACAP+/- mice. At threshold LTP of PACAP+/- mice was impaired greater than the one of PAC1R-/- mice. Thus, both PACAP and PAC1R could contribute to the establishment of LTP in a gene dosage-dependent manner, although PACAP rather than PAC1R might play a pivotal role in learning and memory function.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Action Potentials / genetics
Animals
Dentate Gyrus / metabolism*
Dentate Gyrus / physiopathology
Electric Stimulation
Gene Dosage
Heterozygote
Homozygote
Learning / physiology
Long-Term Potentiation / genetics*
Memory / physiology
Memory Disorders / genetics
Memory Disorders / metabolism
Mice
Mice, Knockout
Mice, Mutant Strains
Neural Pathways / physiopathology
Neurons / physiology
Neuropeptides / deficiency*
Neuropeptides / genetics
Pituitary Adenylate Cyclase-Activating Polypeptide
Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide
Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I
Receptors, Pituitary Hormone / deficiency*
Receptors, Pituitary Hormone / genetics
Synaptic Transmission / genetics

Substances

Adcyap1 protein, mouse
Adcyap1r1 protein, mouse
Neuropeptides
Pituitary Adenylate Cyclase-Activating Polypeptide
Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide
Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I
Receptors, Pituitary Hormone